IMMUNOLOGICAL TECHNIQUES QUIZ

The competitive immunoassay can be used

to detect antibody associated with allergies (IgE)

to detect very small amounts of antigen

both (a) and (b)

commonly to detect trace amounts of drugs.

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Which of the following is an immunoelectrophoresis technique?

Counterimmunoelectrophoresis

Coimmunoelectrophoresis

Two-dimensional immunoelectrophoresis

Both (a) and (b)

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An antigen preparation and an antibody preparation are tested by immunodiffusion. Three bands are found, indicating that

there was one antibody and one antigen

the antibody was impure

the temperature was too high

there was more than one antigen

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In Counter immunoelectrophoresis

the antibody will migrate towards cathode

electrophoresis will drive the antibody and antigen parallel to each other

the antibody will migrate towards anode

electrophoresis will drive the antibody and antigen toward each other

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Agarose a porous matrix used in various molecular biology technique is made by repetitive units of

agarobiose

none of these

maltose

agar

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The IPTG is a gratuitous inducers of (β-galactosidase, and x-gal is a chromogenic substrate. When α-complementation occurs which colour is produced?

Blue

No colour is produced

Brown

White

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DNA- footprinting is a suitable technique for identifying which of the following?

Particular mRNA in mixture

Introns within DNA

Protein binding site within DNA

Particular t-RNA in mixture

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Agarose gel electrophoresis and pulsed field gel electrophoresis may be used to resolve respectively

10 kb and 1000 kb DNA

1000 kb and l0kb DNA

20 kb and 2000 kb DNA

2000 kb and 20kb DNA

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RAST test (radio allergosorbent test) is often used to detect

None of these

bacteriophages

antibodies associated with allergies (IgE)

antigen associated with allergies (IgE)

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Radial Immunodiffusion is similar to

Ouchterloney technique

all of these

gel diffusion

double-diffusion

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