IMMUNOLOGICAL TECHNIQUES QUIZ

What radioactive molecule is used to end-label an oligonucleotide?

Any of these

(alpha)-32P-dUTP

(gamma)-32P-ATP

(alpha)-32P-ATP

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RAST test (radio allergosorbent test) is often used to detect

antibodies associated with allergies (IgE)

bacteriophages

None of these

antigen associated with allergies (IgE)

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In Rocket Immunodiffusion the length of the rocket is

proportional to the amount of antibody placed in each well

proportional to the amount of antigen placed in each well

inversely proportional to the amount of antibody placed in each well

inversely proportional to the amount of antigen placed in each well

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Rocket Immunodiffusion is also known as

gel diffusion

electroimmunodiffusion

none of these

double-diffusion

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Agarose gel electrophoresis and pulsed field gel electrophoresis may be used to resolve respectively

10 kb and 1000 kb DNA

2000 kb and 20kb DNA

20 kb and 2000 kb DNA

1000 kb and l0kb DNA

TRUE ANSWER : ?
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The competitive immunoassay can be used

both (a) and (b)

commonly to detect trace amounts of drugs.

to detect very small amounts of antigen

to detect antibody associated with allergies (IgE)

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An antigen preparation and an antibody preparation are tested by immunodiffusion. Three bands are found, indicating that

the temperature was too high

the antibody was impure

there was more than one antigen

there was one antibody and one antigen

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Agarose a porous matrix used in various molecular biology technique is made by repetitive units of

agar

agarobiose

maltose

none of these

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Which of the following is an immunoelectrophoresis technique?

Counterimmunoelectrophoresis

Coimmunoelectrophoresis

Both (a) and (b)

Two-dimensional immunoelectrophoresis

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Different form of a plasmid, on an agarose gel, after electrophoresis may be in which of the following sequence in decreasing molecular weight?

Open circular, super coiled, linear plasmid DNA

Open circular, linear plasmid, super coiled plasmid

Open circular, super coiled, linear plasmid DNA

Linear plasmid, open circular, super coiled plasmid

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