IMMUNOLOGICAL TECHNIQUES QUIZ

Agarose gel electrophoresis and pulsed field gel electrophoresis may be used to resolve respectively

20 kb and 2000 kb DNA

2000 kb and 20kb DNA

10 kb and 1000 kb DNA

1000 kb and l0kb DNA

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What is the ELISA test intended to measure?

Antigen to HIV only

Antibody to HIV only

Antibodies directed against HLA molecules

Presence of free, circulating virus in the patient

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The IPTG is a gratuitous inducers of (β-galactosidase, and x-gal is a chromogenic substrate. When α-complementation occurs which colour is produced?

No colour is produced

White

Brown

Blue

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In Counter immunoelectrophoresis

electrophoresis will drive the antibody and antigen parallel to each other

electrophoresis will drive the antibody and antigen toward each other

the antibody will migrate towards anode

the antibody will migrate towards cathode

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What radioactive molecule is used to end-label an oligonucleotide?

(gamma)-32P-ATP

(alpha)-32P-ATP

(alpha)-32P-dUTP

Any of these

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RAST test (radio allergosorbent test) is often used to detect

antigen associated with allergies (IgE)

bacteriophages

None of these

antibodies associated with allergies (IgE)

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What would happen if the anti-human Ig-conjugate is not washed free of the well before the substrate is added?

None of these

All wells would show uniform over-development due to unbound and excess anti-human Ig enzyme conjugate

The ELISA would develop normally

The ELISA would not develop when the substrate was added

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Which of the following is an immunodiffusion test?

All of these

Double-diffusion

Ouchterloney technique

Gel diffusion

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Agarose a porous matrix used in various molecular biology technique is made by repetitive units of

agarobiose

agar

none of these

maltose

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Different form of a plasmid, on an agarose gel, after electrophoresis may be in which of the following sequence in decreasing molecular weight?

Open circular, super coiled, linear plasmid DNA

Linear plasmid, open circular, super coiled plasmid

Open circular, linear plasmid, super coiled plasmid

Open circular, super coiled, linear plasmid DNA

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