IMMUNOLOGICAL TECHNIQUES QUIZ

The competitive immunoassay can be used

to detect antibody associated with allergies (IgE)

both (a) and (b)

to detect very small amounts of antigen

commonly to detect trace amounts of drugs.

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Which of the following is a positively charged matrix for ion exchange chromatography?

Phosphocellulose

CM cellulose

None of these

DEAE cellulose

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Agarose a porous matrix used in various molecular biology technique is made by repetitive units of

maltose

agarobiose

none of these

agar

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In Rocket Immunodiffusion the length of the rocket is

inversely proportional to the amount of antigen placed in each well

proportional to the amount of antigen placed in each well

proportional to the amount of antibody placed in each well

inversely proportional to the amount of antibody placed in each well

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Immunoelectrophoresis techniques are designed to separate the mixture components from each other by using electrophoresis

after reaction with antibody

prior to reaction with antigen

after reaction with antigen

prior to reaction with antibody

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Different form of a plasmid, on an agarose gel, after electrophoresis may be in which of the following sequence in decreasing molecular weight?

Linear plasmid, open circular, super coiled plasmid

Open circular, super coiled, linear plasmid DNA

Open circular, linear plasmid, super coiled plasmid

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What is the ELISA test intended to measure?

Antigen to HIV only

Presence of free, circulating virus in the patient

Antibodies directed against HLA molecules

Antibody to HIV only

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In Counter immunoelectrophoresis

electrophoresis will drive the antibody and antigen parallel to each other

the antibody will migrate towards cathode

electrophoresis will drive the antibody and antigen toward each other

the antibody will migrate towards anode

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What would happen if serum is omitted from the ELISA, but all other steps remain same?

Anti-human Ig-conjugate would not bind and be washed away

Both (a) and (b)

The OD values would be nearly the same as the assay control

Anti-human Ig-conjugate would bind non-specifically to the ELISA plate

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Immunoaffinity chromatography can be used in biochemical applications to

purify protein antigen

break down antigen and analyze quantitatively

break down antibody structure

None of these

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