IMMUNOLOGICAL TECHNIQUES QUIZ

double-diffusion

gel diffusion

none of these

electroimmunodiffusion

Antigen to HIV only

Presence of free, circulating virus in the patient

Antibody to HIV only

Antibodies directed against HLA molecules

the antibody was impure

there was one antibody and one antigen

the temperature was too high

there was more than one antigen

Coimmunoelectrophoresis

Both (a) and (b)

Two-dimensional immunoelectrophoresis

Counterimmunoelectrophoresis

Both (a) and (b)

The OD values would be nearly the same as the assay control

Anti-human Ig-conjugate would not bind and be washed away

Anti-human Ig-conjugate would bind non-specifically to the ELISA plate

commonly to detect trace amounts of drugs.

to detect very small amounts of antigen

to detect antibody associated with allergies (IgE)

both (a) and (b)

Linear plasmid, open circular, super coiled plasmid

Open circular, super coiled, linear plasmid DNA

Open circular, linear plasmid, super coiled plasmid

Open circular, super coiled, linear plasmid DNA

(gamma)-32P-ATP

(alpha)-32P-dUTP

(alpha)-32P-ATP

Any of these

electrophoresis will drive the antibody and antigen parallel to each other

the antibody will migrate towards cathode

electrophoresis will drive the antibody and antigen toward each other

the antibody will migrate towards anode

None of these

CM cellulose

Phosphocellulose

DEAE cellulose