IMMUNE RESPONSE IN BIOCHEMISTRY QUIZ

What useful information can be found from a Van Deemter plot?

The selectivity factor

Optimum column length

Optimum column temperature

Optimum mobile phase flow rate

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Resolution is proportional to the

square of the number of theoretical plates in a column

square root of the number of theoretical plates in a column

number of theoretical plates in a column

cube root of the number of theoretical plates in a column

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Derivatisation of a sample is carried out to

increase volatility of the analytes

reduce polarity of the analytes

increase the detector response

All of these

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What are the benefits of decreasing the column internal diameter?

All of these

Increased sample capacity

Reduced risk of column overloading

Increased resolution

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The GC trace obtained after an experiment is called a

graph

chromatograph

chromatophore

chromatogram

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Which of the statements is correct?

Gas chromatography is used to analyse gases

Gas chromatography is used to analyse solids

All of these

Gas chromatography is used to analyse gases, solutions and solids

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Which of these effects result from slow injection of a large sample volume?

Increased resolution

Decreased resolution

Non-linear detector response

Constant resolution

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What does the retention factor, k', describe?

The distribution of an analyte between the stationary and the mobile phase

The velocity of the mobile phase

All of these

The migration rate of an analyte through a column

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Doubling the column's length increases resolution by a factor of

(2)0.5

4

2

3

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Sample retention in the column is measured by

retention index

retention time

all of these

retention factor

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