GENE TRANSFER IN PLANTS QUIZ

transfer sequences

border sequences

cos site

flanking sequences

Integration of T DNA can occur at many different, apparently random, sites in the plant nuclear DNA

Integration of T DNA occurs only at one specific sites in the plant nuclear DNA

Integration of T DNA occurs at two specific sites in the plant nuclear DNA

Integration of T DNA occurs at one site that may be random in the plant nuclear DNA

injection of large amount of DNA

All of these

injection of DNA into bigger cells

injection with needle having diameter greater than cell diameter

Both (a) and (b)

α-hydroxy syringone

None of these

Acetosyringone

140-235 kb

80-120 kb

>235 kb

40-80 kb

all of these

enzymes for agropine biosynthesis

conjugative transfer

tumour formation

intact cells are used

method is universal in its application irrespective of all shape, size, type and presence or absence of cell wall

All of these

gene can be transferred to many cells simultaneously

vir B and vir E

vir C and vir D

vir A and vir B

vir A and vir G

pRK 2013

pBR 322 based plasmid vector

pRN 3

all of these

a yeast

gram (+) bacteria

gram (-) bacteria

a fungi