GEL ELECTROPHORESIS QUIZ

both (a) and (b)

smaller proteins enter the beads more readily

large proteins enter the beads more readily

large proteins elute first

positively charged side chains

negatively charged side chains

molecular weight

different isoelectric points

treated with a oxidizing agent and then with anionic detergent followed by fractionation by electrophoresis

fractionated by electrophoresis then treated with an oxidizing agent followed by anionic detergent.

treated with a reducing agent and then with anionic detergent followed by fractionation by electrophoresis

None of these

both proteins migrate to the anode

histones migrate to the anode and myoglobin migrates to the cathode

histones migrate to the cathode and myoglobin migrates to the anode

both proteins migrate to the cathode

staining them with the dye

measuring their molecular weight

none of these

using electron microscope only

size

relative content of positively charged residue only

relative content of negatively charged residue only

relative content of positively and negatively charged residue

combining information from (a)and (b)

isoelectric focusing

SDS-PAGE electrophoresis

gel filtration chromatography

net charge and size

net negative charge

net positive charge

net positive charges size

proteins have the same charge-to-mass ratio

proteins are denatured by the SDS

smaller proteins migrate more rapidly through the gel

All of these