Proteins are separated in an SDS-PAGE experiment on the basis of their negatively charged side chains molecular weight different isoelectric points positively charged side chains TRUE ANSWER : ? YOUR ANSWER : ?
In SDS-PAGE, the protein sample is first treated with a reducing agent and then with anionic detergent followed by fractionation by electrophoresis None of these fractionated by electrophoresis then treated with an oxidizing agent followed by anionic detergent. treated with a oxidizing agent and then with anionic detergent followed by fractionation by electrophoresis TRUE ANSWER : ? YOUR ANSWER : ?
In a native PAGE, proteins are separated on the basis of net positive charge net negative charge net positive charges size net charge and size TRUE ANSWER : ? YOUR ANSWER : ?
Electrophoresis of histones and myoglobin under non-denaturing conditions (pH = 7.0) results in histones migrate to the cathode and myoglobin migrates to the anode both proteins migrate to the anode both proteins migrate to the cathode histones migrate to the anode and myoglobin migrates to the cathode TRUE ANSWER : ? YOUR ANSWER : ?
In a gel filtration column large proteins enter the beads more readily both (a) and (b) smaller proteins enter the beads more readily large proteins elute first TRUE ANSWER : ? YOUR ANSWER : ?
In an SDS-PAGE smaller proteins migrate more rapidly through the gel proteins are denatured by the SDS proteins have the same charge-to-mass ratio All of these TRUE ANSWER : ? YOUR ANSWER : ?
In isoelectric focusing, proteins are separated on the basis of their relative content of positively and negatively charged residue relative content of negatively charged residue only size relative content of positively charged residue only TRUE ANSWER : ? YOUR ANSWER : ?
Proteins can be visualized directly in gels by staining them with the dye none of these using electron microscope only measuring their molecular weight TRUE ANSWER : ? YOUR ANSWER : ?
The subunit molecular weight as well as the number of subunits in the quaternary structure can be determined by combining information from (a)and (b) gel filtration chromatography isoelectric focusing SDS-PAGE electrophoresis TRUE ANSWER : ? YOUR ANSWER : ?