GEL ELECTROPHORESIS QUIZ

positively charged side chains

molecular weight

different isoelectric points

negatively charged side chains

net charge and size

net negative charge

net positive charge

net positive charges size

large proteins enter the beads more readily

large proteins elute first

smaller proteins enter the beads more readily

both (a) and (b)

staining them with the dye

using electron microscope only

none of these

measuring their molecular weight

histones migrate to the anode and myoglobin migrates to the cathode

both proteins migrate to the anode

both proteins migrate to the cathode

histones migrate to the cathode and myoglobin migrates to the anode

combining information from (a)and (b)

SDS-PAGE electrophoresis

isoelectric focusing

gel filtration chromatography

All of these

smaller proteins migrate more rapidly through the gel

proteins are denatured by the SDS

proteins have the same charge-to-mass ratio

relative content of negatively charged residue only

size

relative content of positively and negatively charged residue

relative content of positively charged residue only

fractionated by electrophoresis then treated with an oxidizing agent followed by anionic detergent.

treated with a oxidizing agent and then with anionic detergent followed by fractionation by electrophoresis

treated with a reducing agent and then with anionic detergent followed by fractionation by electrophoresis

None of these