GEL ELECTROPHORESIS QUIZ

proteins are denatured by the SDS

All of these

proteins have the same charge-to-mass ratio

smaller proteins migrate more rapidly through the gel

SDS-PAGE electrophoresis

combining information from (a)and (b)

isoelectric focusing

gel filtration chromatography

None of these

treated with a reducing agent and then with anionic detergent followed by fractionation by electrophoresis

treated with a oxidizing agent and then with anionic detergent followed by fractionation by electrophoresis

fractionated by electrophoresis then treated with an oxidizing agent followed by anionic detergent.

histones migrate to the anode and myoglobin migrates to the cathode

both proteins migrate to the cathode

histones migrate to the cathode and myoglobin migrates to the anode

both proteins migrate to the anode

positively charged side chains

molecular weight

negatively charged side chains

different isoelectric points

net charge and size

net positive charges size

net positive charge

net negative charge

smaller proteins enter the beads more readily

both (a) and (b)

large proteins elute first

large proteins enter the beads more readily

using electron microscope only

none of these

staining them with the dye

measuring their molecular weight

relative content of positively charged residue only

relative content of positively and negatively charged residue

size

relative content of negatively charged residue only