GEL ELECTROPHORESIS QUIZ

histones migrate to the anode and myoglobin migrates to the cathode

both proteins migrate to the anode

histones migrate to the cathode and myoglobin migrates to the anode

both proteins migrate to the cathode

positively charged side chains

negatively charged side chains

different isoelectric points

molecular weight

large proteins elute first

large proteins enter the beads more readily

both (a) and (b)

smaller proteins enter the beads more readily

net positive charge

net charge and size

net positive charges size

net negative charge

relative content of positively charged residue only

size

relative content of positively and negatively charged residue

relative content of negatively charged residue only

treated with a reducing agent and then with anionic detergent followed by fractionation by electrophoresis

None of these

fractionated by electrophoresis then treated with an oxidizing agent followed by anionic detergent.

treated with a oxidizing agent and then with anionic detergent followed by fractionation by electrophoresis

SDS-PAGE electrophoresis

isoelectric focusing

combining information from (a)and (b)

gel filtration chromatography

none of these

using electron microscope only

measuring their molecular weight

staining them with the dye

proteins have the same charge-to-mass ratio

proteins are denatured by the SDS

smaller proteins migrate more rapidly through the gel

All of these