GEL ELECTROPHORESIS QUIZ

relative content of positively charged residue only

size

relative content of negatively charged residue only

relative content of positively and negatively charged residue

both proteins migrate to the anode

histones migrate to the anode and myoglobin migrates to the cathode

histones migrate to the cathode and myoglobin migrates to the anode

both proteins migrate to the cathode

molecular weight

different isoelectric points

negatively charged side chains

positively charged side chains

isoelectric focusing

gel filtration chromatography

combining information from (a)and (b)

SDS-PAGE electrophoresis

net positive charge

net positive charges size

net negative charge

net charge and size

staining them with the dye

using electron microscope only

none of these

measuring their molecular weight

proteins have the same charge-to-mass ratio

proteins are denatured by the SDS

smaller proteins migrate more rapidly through the gel

All of these

large proteins elute first

both (a) and (b)

smaller proteins enter the beads more readily

large proteins enter the beads more readily

treated with a reducing agent and then with anionic detergent followed by fractionation by electrophoresis

fractionated by electrophoresis then treated with an oxidizing agent followed by anionic detergent.

None of these

treated with a oxidizing agent and then with anionic detergent followed by fractionation by electrophoresis