GEL ELECTROPHORESIS QUIZ

treated with a oxidizing agent and then with anionic detergent followed by fractionation by electrophoresis

None of these

fractionated by electrophoresis then treated with an oxidizing agent followed by anionic detergent.

treated with a reducing agent and then with anionic detergent followed by fractionation by electrophoresis

combining information from (a)and (b)

gel filtration chromatography

SDS-PAGE electrophoresis

isoelectric focusing

net positive charge

net charge and size

net positive charges size

net negative charge

none of these

using electron microscope only

measuring their molecular weight

staining them with the dye

size

relative content of negatively charged residue only

relative content of positively charged residue only

relative content of positively and negatively charged residue

molecular weight

positively charged side chains

different isoelectric points

negatively charged side chains

All of these

proteins have the same charge-to-mass ratio

smaller proteins migrate more rapidly through the gel

proteins are denatured by the SDS

large proteins elute first

both (a) and (b)

large proteins enter the beads more readily

smaller proteins enter the beads more readily

both proteins migrate to the anode

histones migrate to the anode and myoglobin migrates to the cathode

both proteins migrate to the cathode

histones migrate to the cathode and myoglobin migrates to the anode