Proteins are separated in an SDS-PAGE experiment on the basis of their positively charged side chains molecular weight different isoelectric points negatively charged side chains TRUE ANSWER : ? YOUR ANSWER : ?
In a native PAGE, proteins are separated on the basis of net charge and size net negative charge net positive charge net positive charges size TRUE ANSWER : ? YOUR ANSWER : ?
In a gel filtration column large proteins enter the beads more readily large proteins elute first smaller proteins enter the beads more readily both (a) and (b) TRUE ANSWER : ? YOUR ANSWER : ?
Proteins can be visualized directly in gels by staining them with the dye using electron microscope only none of these measuring their molecular weight TRUE ANSWER : ? YOUR ANSWER : ?
Electrophoresis of histones and myoglobin under non-denaturing conditions (pH = 7.0) results in histones migrate to the anode and myoglobin migrates to the cathode both proteins migrate to the anode both proteins migrate to the cathode histones migrate to the cathode and myoglobin migrates to the anode TRUE ANSWER : ? YOUR ANSWER : ?
The subunit molecular weight as well as the number of subunits in the quaternary structure can be determined by combining information from (a)and (b) SDS-PAGE electrophoresis isoelectric focusing gel filtration chromatography TRUE ANSWER : ? YOUR ANSWER : ?
In an SDS-PAGE All of these smaller proteins migrate more rapidly through the gel proteins are denatured by the SDS proteins have the same charge-to-mass ratio TRUE ANSWER : ? YOUR ANSWER : ?
In isoelectric focusing, proteins are separated on the basis of their relative content of negatively charged residue only size relative content of positively and negatively charged residue relative content of positively charged residue only TRUE ANSWER : ? YOUR ANSWER : ?
In SDS-PAGE, the protein sample is first fractionated by electrophoresis then treated with an oxidizing agent followed by anionic detergent. treated with a oxidizing agent and then with anionic detergent followed by fractionation by electrophoresis treated with a reducing agent and then with anionic detergent followed by fractionation by electrophoresis None of these TRUE ANSWER : ? YOUR ANSWER : ?