GEL ELECTROPHORESIS QUIZ

staining them with the dye

measuring their molecular weight

none of these

using electron microscope only

SDS-PAGE electrophoresis

gel filtration chromatography

isoelectric focusing

combining information from (a)and (b)

both proteins migrate to the anode

histones migrate to the anode and myoglobin migrates to the cathode

both proteins migrate to the cathode

histones migrate to the cathode and myoglobin migrates to the anode

negatively charged side chains

positively charged side chains

molecular weight

different isoelectric points

proteins are denatured by the SDS

proteins have the same charge-to-mass ratio

All of these

smaller proteins migrate more rapidly through the gel

net charge and size

net positive charges size

net negative charge

net positive charge

None of these

treated with a reducing agent and then with anionic detergent followed by fractionation by electrophoresis

treated with a oxidizing agent and then with anionic detergent followed by fractionation by electrophoresis

fractionated by electrophoresis then treated with an oxidizing agent followed by anionic detergent.

relative content of positively and negatively charged residue

size

relative content of positively charged residue only

relative content of negatively charged residue only

both (a) and (b)

smaller proteins enter the beads more readily

large proteins elute first

large proteins enter the beads more readily