GEL ELECTROPHORESIS QUIZ

smaller proteins enter the beads more readily

large proteins enter the beads more readily

large proteins elute first

both (a) and (b)

both proteins migrate to the cathode

histones migrate to the anode and myoglobin migrates to the cathode

histones migrate to the cathode and myoglobin migrates to the anode

both proteins migrate to the anode

fractionated by electrophoresis then treated with an oxidizing agent followed by anionic detergent.

treated with a oxidizing agent and then with anionic detergent followed by fractionation by electrophoresis

treated with a reducing agent and then with anionic detergent followed by fractionation by electrophoresis

None of these

net negative charge

net positive charge

net charge and size

net positive charges size

smaller proteins migrate more rapidly through the gel

proteins are denatured by the SDS

proteins have the same charge-to-mass ratio

All of these

combining information from (a)and (b)

gel filtration chromatography

isoelectric focusing

SDS-PAGE electrophoresis

relative content of positively and negatively charged residue

size

relative content of negatively charged residue only

relative content of positively charged residue only

staining them with the dye

none of these

measuring their molecular weight

using electron microscope only

different isoelectric points

positively charged side chains

molecular weight

negatively charged side chains