Which of the following is the basis of first dimension of separation for two-dimensional electrophoresis? Folding Molecular mass Isoelectric point Solubility TRUE ANSWER : ? YOUR ANSWER : ?
The sequence of amino acids in proteins can be determined by means of identification of the -COOH terminal amino acids identification of the -NH2 terminal amino acids All of these partial cleavage of the original polypeptide into smaller polypeptides TRUE ANSWER : ? YOUR ANSWER : ?
How many possible orientations do spin 1/2 nuclei have when they are located in an applied magnetic field? 2 6 3 4 TRUE ANSWER : ? YOUR ANSWER : ?
What is the name given to the relaxation process due to an interaction between an excited nucleus and the magnetic fields caused by nuclei in molecules moving around in the sample? Spin - spin relaxation None of these Spin - spin - spin relaxation Spin - lattice relaxation TRUE ANSWER : ? YOUR ANSWER : ?
What is meant by rotating frame of reference? None of these That the detector rotates around the sample That the sample is spun rapidly in the applied field If the laboratory itself is imagined to be rotated at the Larmor frequency, viewing that individual magnetic moment vectors are fixed in space TRUE ANSWER : ? YOUR ANSWER : ?
When radiation energy is absorbed by a spin 1/2 nucleus in a magnetic field, what happens? The processional frequency of the nucleus increases The angle of precession flips so that the magnetic moment of the nucleus opposes the applied field None of these The nucleus spins faster TRUE ANSWER : ? YOUR ANSWER : ?
Why is it advantageous to record many FID signals from the same sample and then add them together? None of these To ensure that all target nuclei in the sample have been excited To increase sensitivity To remove inaccuracies caused by fluctuations in the applied magnetic field TRUE ANSWER : ? YOUR ANSWER : ?
In FT-NMR, how are nuclei excited? By radio-frequency radiation whose frequency is swept across a predetermined range By an intense pulse of radiation which contains a wide range of frequencies By an intense pressure None of these TRUE ANSWER : ? YOUR ANSWER : ?
In scanning electron microscopy a specimen is fixed and then coated with thin layer of a heavy metal None of these a specimen is not fixed and then coated with thin layer of a heavy metal a specimen is fixed and then coated with transition metal TRUE ANSWER : ? YOUR ANSWER : ?