The best way to determine the location of protein in the purification scheme is to measure the rate of ATP synthesis changes in the refractive index mass spectroscopy of the protein UV absorption TRUE ANSWER : ? YOUR ANSWER : ?
A purified protein sample contains 10 μg of protein and has an enzyme activity of 1 m mole of ATP synthesized/sec (1 unit). What is the specific activity of the final purified sample? 100,000 units/mg 10,000 units/mg. 1,000 units/mg 1,000,000 units/mg TRUE ANSWER : ? YOUR ANSWER : ?
Proteins separation can be carried out on the basis of solubility in salt solutions net charge all of these size or mass TRUE ANSWER : ? YOUR ANSWER : ?
In ion-exchange chromatography proteins are separated on the basis of their net charge proteins are separated on the basis of their size either (b) or (c) proteins are separated on the basis of their shape TRUE ANSWER : ? YOUR ANSWER : ?
Which of the following may be added to stabilize the protein after yeast cells disruption? All of these NaCl Protease inhibitor AMP TRUE ANSWER : ? YOUR ANSWER : ?
Gel-filtration chromatography separates on the basis of shape using porous beads packed in a column size and shape using porous beads packed in a column None of these size using porous beads packed in a column TRUE ANSWER : ? YOUR ANSWER : ?
During successful purification scheme, this may be expected that the specific activity decreases both (a) and (c) specific activity increases number of proteins in the sample decreases TRUE ANSWER : ? YOUR ANSWER : ?
Affinity chromatography deals with the protein - protein interaction specific binding of a protein constituents for another molecule None of these protein - carbohydrate interaction TRUE ANSWER : ? YOUR ANSWER : ?