Which of the following statements about enzymes or their function is true? Enzymes are proteins whose three-dimensional form is key to their function Enzymes speed up reactions by lowering activation energy Enzymes do not alter the overall change in free energy for a reaction All of these TRUE ANSWER : ? YOUR ANSWER : ?
The proteolysis rate enhancement by chymotrypsin (~1010 folds) corresponds to a reduction in activation energy of about 40 kJ/mol 88 kJ/mol 58 kJ/mol 49 kJ/mol TRUE ANSWER : ? YOUR ANSWER : ?
Which of the following is false statement with regard to comparison between Serine and HIV proteases? Both forms an acyl-enzyme intermediate Both require water to complete the catalytic cycle Both show specificity for certain amino acid sequences Both use nucleophilic attack to hydrolyze the peptide bond TRUE ANSWER : ? YOUR ANSWER : ?
Common feature in all serine proteases is a hydrophobic specificity pocket cluster of reactive serine residues hydrophilic specificity pocket single reactive serine residue TRUE ANSWER : ? YOUR ANSWER : ?
The role of Asp 102 and His 57 during trypsin catalysis is to neutralize the charge on the other's side chain clamp the substrate into the active site function as a proton shuttle keep the specificity pocket open TRUE ANSWER : ? YOUR ANSWER : ?
Tryprotophan synthetase of E.coli, a typical bifunctional oligomeric enzyme consist of a protein designated B two proteins designated A and B a protein designated A a protein A and one-subunit a TRUE ANSWER : ? YOUR ANSWER : ?
In the enzyme-catalyzed reaction shown below, what will be the effect on substances A, B, C, and D of inactivating the enzyme labeled E2? A ---(E1)---> B ---(E2)---> C ---(E3)---> A, B, C, and D will all still be produced A, B, and C will still be produced, but not D A will still be produced, but not B, C, or D A and B will still be produced, but not C or D TRUE ANSWER : ? YOUR ANSWER : ?
Which of the common features are shared between serine and aspartate proteases? Both require water to complete the catalytic cycle All of these Both use a base to activate the nucleophile Both show specificity for certain amino acid sequences TRUE ANSWER : ? YOUR ANSWER : ?
The cleavage specificity of trypsin and chymotrypsin depend in part on the size, shape, and charge of the active site or specificity pocket absence of water in the active site presence of a low-barrier hydrogen bond in the active site or specificity pocket proximity of Ser 195 to the active site or specificity pocket TRUE ANSWER : ? YOUR ANSWER : ?
How is the enzyme COX-1 important in human health? It catalyzes the production of hormones that maintain the stomach lining It is critical for the biosynthesis of DNA It helps to transport carbon dioxide in the blood It is a chemical derivative of aspirin TRUE ANSWER : ? YOUR ANSWER : ?