Which of the following is false statement with regard to comparison between Serine and HIV proteases? Both use nucleophilic attack to hydrolyze the peptide bond Both require water to complete the catalytic cycle Both forms an acyl-enzyme intermediate Both show specificity for certain amino acid sequences TRUE ANSWER : ? YOUR ANSWER : ?
The proteolysis rate enhancement by chymotrypsin (~1010 folds) corresponds to a reduction in activation energy of about 88 kJ/mol 58 kJ/mol 40 kJ/mol 49 kJ/mol TRUE ANSWER : ? YOUR ANSWER : ?
In the enzyme-catalyzed reaction shown below, what will be the effect on substances A, B, C, and D of inactivating the enzyme labeled E2? A ---(E1)---> B ---(E2)---> C ---(E3)---> A will still be produced, but not B, C, or D A, B, and C will still be produced, but not D A, B, C, and D will all still be produced A and B will still be produced, but not C or D TRUE ANSWER : ? YOUR ANSWER : ?
The role of Asp 102 and His 57 during trypsin catalysis is to keep the specificity pocket open clamp the substrate into the active site function as a proton shuttle neutralize the charge on the other's side chain TRUE ANSWER : ? YOUR ANSWER : ?
The cleavage specificity of trypsin and chymotrypsin depend in part on the absence of water in the active site proximity of Ser 195 to the active site or specificity pocket size, shape, and charge of the active site or specificity pocket presence of a low-barrier hydrogen bond in the active site or specificity pocket TRUE ANSWER : ? YOUR ANSWER : ?
Common feature in all serine proteases is a single reactive serine residue hydrophobic specificity pocket hydrophilic specificity pocket cluster of reactive serine residues TRUE ANSWER : ? YOUR ANSWER : ?
Before they can react, many molecules need to be destabilized. This state is typically achieved through changing the reaction from a biosynthetic to a catabolic pathway the input of a small amount of activation energy changing the three-dimensional shape of the molecule oxidizing the molecules by removing electrons TRUE ANSWER : ? YOUR ANSWER : ?
Tryprotophan synthetase of E.coli, a typical bifunctional oligomeric enzyme consist of two proteins designated A and B a protein A and one-subunit a a protein designated A a protein designated B TRUE ANSWER : ? YOUR ANSWER : ?
What is the specificity of the Clostripain protease? It cleave after Lys residues None of these It cleave after Arg residues It cleave after His residues TRUE ANSWER : ? YOUR ANSWER : ?
The nucleophile in serine proteases is Asparagine Serine both (a) and (b) water TRUE ANSWER : ? YOUR ANSWER : ?