The role of Asp 102 and His 57 during trypsin catalysis is to neutralize the charge on the other's side chain function as a proton shuttle keep the specificity pocket open clamp the substrate into the active site TRUE ANSWER : ? YOUR ANSWER : ?
Before they can react, many molecules need to be destabilized. This state is typically achieved through changing the three-dimensional shape of the molecule oxidizing the molecules by removing electrons changing the reaction from a biosynthetic to a catabolic pathway the input of a small amount of activation energy TRUE ANSWER : ? YOUR ANSWER : ?
The nucleophile in serine proteases is water both (a) and (b) Serine Asparagine TRUE ANSWER : ? YOUR ANSWER : ?
In the enzyme-catalyzed reaction shown below, what will be the effect on substances A, B, C, and D of inactivating the enzyme labeled E2? A ---(E1)---> B ---(E2)---> C ---(E3)---> A, B, and C will still be produced, but not D A will still be produced, but not B, C, or D A and B will still be produced, but not C or D A, B, C, and D will all still be produced TRUE ANSWER : ? YOUR ANSWER : ?
The E.coli pyruvic acid dehydrogenase complex is reported to decatalyze the oxidation of pyruvic acid to acetyl Co A and CO2 Catalyze the oxidation of pyruvic acid to acetyl Co A and CO2 Catalyze the reduction of pyruvic acid to acetyl Co A and CO2 retard the reduction of pyruvic acid to acetyl Co A and CO2 TRUE ANSWER : ? YOUR ANSWER : ?
Which of the following is false statement with regard to comparison between Serine and HIV proteases? Both show specificity for certain amino acid sequences Both forms an acyl-enzyme intermediate Both use nucleophilic attack to hydrolyze the peptide bond Both require water to complete the catalytic cycle TRUE ANSWER : ? YOUR ANSWER : ?
What is the specificity of the Clostripain protease? It cleave after Lys residues It cleave after His residues None of these It cleave after Arg residues TRUE ANSWER : ? YOUR ANSWER : ?
Which of the following (s) is/are serine proteases? Elastase Chymotrypsin Trypsin all of these TRUE ANSWER : ? YOUR ANSWER : ?
Enzyme-driven metabolic pathways can be made more efficient by All of these concentrating enzymes within specific cellular compartments fixing enzymes into membranes so that they are adjacent to each other grouping enzymes into free-floating, multienzyme complexes TRUE ANSWER : ? YOUR ANSWER : ?
The cleavage specificity of trypsin and chymotrypsin depend in part on the size, shape, and charge of the active site or specificity pocket absence of water in the active site proximity of Ser 195 to the active site or specificity pocket presence of a low-barrier hydrogen bond in the active site or specificity pocket TRUE ANSWER : ? YOUR ANSWER : ?