DNA STRUCTURE AND REPLICATION QUIZ

A cesium chloride will separate DNA molecules by

adhesion

resorption

density

absorption

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What is the name given to the points at which a DNA helix is unwound and new strands develop?

Leading strands

Okazaki fragments

Replication origins

Replication forks

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The chromosomal DNA complexes with

two types of histone as H1 and H4

four types of histone as H1, H2A, H3 and H4

five types of histone as H1, H2A, H2B, H3 and H4

three types of histone as H1, H2A and H4

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Replication in E. coli is initiated by the generation of short RNA primers using

RNA polymerase

both (a) and (b)

reverse transcriptase

primase

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When DNA polymerase is in contact with guanine in the parental strand, what does it add to the growing daughter strand?

Phosphate

Cytosine

Uracil

Guanine

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Automated DNA sequencing uses the chain termination method

with no primer

either (b) or (c)

but with a polynucleotide primer labeled with a fluorescent dye

but with an oligonucleotide primer labeled with a fluorescent dye

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If one cell has AT contents 40%, what will be the percentage of Guanine residue?

Guanine residue can not be calculated

60%

15%

30%

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Which histone(s) are associated with the linker DNA of a nucleosome?

Histone H1

Histone H4

Histone H3

Histones H2A and H2B

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In DNA replication, one new strand is synthesized complementary to an old (parent) strand and is referred as

antiparallel

semiconservative

discontinuous

mutational

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The size of a linear DNA fragment in a gel can be estimated by

the time it takes the gel to run

the sedimentation coefficient

fluorescence in UV light

comparison with a molecular weight standard such as Hind III digested phage lambda

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