DNA STRUCTURE AND REPLICATION QUIZ

The enzyme responsible for breaking the hydrogen bonds, and thus separating the DNA strands during DNA synthesis is

helicase

none of these

strandase

DNA polymerase

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E.coli DNA polymerases II and III lack

5' → 3' exonuclease activity

partially 5' → 3' endonuclease activity

5' → 3' endonuclease activity

partially 5' → 3' exonuclease activity

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What is the exact name of the classical Watson-Crick double helix DNA?

B-DNA

X-DNA

Z-DNA

A-DNA

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Proteins which help to open the double helix for DNA synthesis are

ligase

DNA helicases

DNA topoisomerase

single stranded binding proteins

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How does ethidium bromide interact with DNA?

It binds to adenine

It binds to phosphate

It intercalates between bases

It does not interact

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One hypothesis of DNA replication suggested that the parental DNA molecules are broken into fragments. Both strands of DNA in each of the daughter molecules are made up of an assortment of parental and new DNA. This statement refers to which hypothesis?

Semi-conservative theory

Dispersive theory

Evolutionary theory

Conservative theory

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Deoxyribonucleoside triphosphates, being need in the chain termination methods are

all of these

dGTP and dCTP

dTTP

dATP

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When DNA polymerase is in contact with guanine in the parental strand, what does it add to the growing daughter strand?

Uracil

Cytosine

Guanine

Phosphate

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DNA replication in eukaryotes occurs only in

G2 phase

G1 phase

M phase

S phase

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The most common liquid volumes in molecular biology are measured in

ml

μl

nl

1

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