DNA SEQUENCING, MUTATION AND REPAIR QUIZ

methodical sequencing a few large cloned fragments of DNA

sequencing the bacterial chromosome while it is still intact

rapidly sequencing thousands of small randomly cloned fragments

All of these

cytosine at start

unique antibody bound to it

distinctive fluorescent tag

different radioactive tag

Arabidopsis chromosome IV

Yeast chromosome III

Yeast chromosome XI

None of these

H. influenzae

E. coli

S. thermophilus

S. pneumoniae

have no effect on the transcript or protein made

shorten the length of the protein encoded by the gene

cause a shift in reading frame

cause premature termination of the mRNA

Incision

Excision

Ligation

All of these

frameshift mutation

missense mutation

all of these

nonsense mutation

nonsense mutation

transversion

none of these

lethal mutation

excision repair

photoreactivation

phosphorylation

photosynthesis

expressed both in bacteria and mammalian cells

a bacterial promoter

the natural promoter of the gene being cloned

not a regulated promoter