DNA REPLICATION QUIZ

DNA gyrase is inhibited by

tetracycline

both (a) and (b)

cephalosporin

nalidixic acid

TRUE ANSWER : ?
YOUR ANSWER : ?

DNA replication rates in prokaryotes are approximately of the order of

1,00 bases per second

1,000 bases per second

10 bases per second

10,000 bases per second

TRUE ANSWER : ?
YOUR ANSWER : ?

Which of the following statements on replication in E.coli is correct?

It occurs in a unidirectional manner

Always uses T7 DNA polymerase when infected by T7 phage

Occurs only when λ phase has infected E. coli

It occurs in bidirectional manner

TRUE ANSWER : ?
YOUR ANSWER : ?

Which of the following lacks 3'-5' exonuclease activity?

All of these

T4 DNA ligase

Short fragment of DNA polymerase I

Taq DNA polymerase

TRUE ANSWER : ?
YOUR ANSWER : ?

Which of the following possesses both 5'-3' and 3'-5' exonuclease activity?

Kornberg enzyme

None of these

Taq DNA polymerase

DNA polymerase III

TRUE ANSWER : ?
YOUR ANSWER : ?

The replication of chromosomes by eukaryotes occurs in a relatively short period of time because

eukaryotic DNA is always single stranded

each chromosome contains multiple replicons

the eukaryotic replication machinery is 1000 times faster than the prokaryotes

the eukaryotes have more amount of DNA for replication

TRUE ANSWER : ?
YOUR ANSWER : ?

In E.coli, which enzyme synthesizes the RNA primer for Okazaki fragments?

DnaC

all of these

DnaA

DnaG

TRUE ANSWER : ?
YOUR ANSWER : ?

The synthesis of DNA by DNA polymerase occurs in the

3' to 5' direction

3' to 3' direction

5' to 3' direction

5' to 5' direction

TRUE ANSWER : ?
YOUR ANSWER : ?

Which of the following repairs nicked DNA by forming a phosphodiester bond between adjacent nucleotides?

DNA gyrase

Topoisomerases

DNA ligase

Helicase

TRUE ANSWER : ?
YOUR ANSWER : ?

Which of the following enzymes unwind short stretches of DNA helix immediately ahead of a replication fork?

Helicases

DNA polymerases

Single-stranded binding proteins

Topoisomerases

TRUE ANSWER : ?
YOUR ANSWER : ?