Gel-filtration chromatography separates on the basis of None of these shape using porous beads packed in a column size and shape using porous beads packed in a column size using porous beads packed in a column TRUE ANSWER : ? YOUR ANSWER : ?
Affinity chromatography deals with the specific binding of a protein constituents for another molecule protein - protein interaction None of these protein - carbohydrate interaction TRUE ANSWER : ? YOUR ANSWER : ?
Which of the following may be added to stabilize the protein after yeast cells disruption? NaCl All of these AMP Protease inhibitor TRUE ANSWER : ? YOUR ANSWER : ?
In ion-exchange chromatography either (b) or (c) proteins are separated on the basis of their size proteins are separated on the basis of their shape proteins are separated on the basis of their net charge TRUE ANSWER : ? YOUR ANSWER : ?
Proteins separation can be carried out on the basis of solubility in salt solutions size or mass all of these net charge TRUE ANSWER : ? YOUR ANSWER : ?
The best way to determine the location of protein in the purification scheme is to measure the UV absorption rate of ATP synthesis changes in the refractive index mass spectroscopy of the protein TRUE ANSWER : ? YOUR ANSWER : ?
During successful purification scheme, this may be expected that the number of proteins in the sample decreases specific activity decreases both (a) and (c) specific activity increases TRUE ANSWER : ? YOUR ANSWER : ?
A purified protein sample contains 10 μg of protein and has an enzyme activity of 1 m mole of ATP synthesized/sec (1 unit). What is the specific activity of the final purified sample? 1,000 units/mg 1,000,000 units/mg 100,000 units/mg 10,000 units/mg. TRUE ANSWER : ? YOUR ANSWER : ?