CLONING VECTORS QUIZ

for probe preparation

for oligonucleotide directed mutagenesis

All of these

for sequencing of cloned DNA

100 kbp

10 kbp

20 kbp

50 kbp

origin of replication

marker genes coding for replication

cleavage site for the insertion of foreign DNA

genes coding for viral proteins

contains cleavage site

helps whole genome in circularization and ligation

both (a) and (b)

consists of 12 bases

None of these

central fragment of the vector DNA

the right arm of the vector DNA

the left arm of the vector DNA

1-2 kb

6-7 kb

30-35 kb

9-20 kb

1-2 kb

15-25 kb

40-44 kb

6-7 kb

50 kb

35-53 kb

40-50 kb

any size

free protein that is secreted out

β-galactosidase fused protein

All of these

free protein in the cytoplasm

bacteriophage

plasmid

phagemid

cosmid