CELL CULTURES AND CHARACTERISTICS QUIZ

Intestinal bacteria can grow in the presence of __________ whereas nonintestinal bacteria are usually inhibited.

sugars

low levels of nitrogen

bile salts

low concentration of various dyes

TRUE ANSWER : ?
YOUR ANSWER : ?

Which of the following methods is most likely to be quantitative?

Gram staining

None of these

Wet mount

Dilution and plating

TRUE ANSWER : ?
YOUR ANSWER : ?

Which of the following chemotherapeutic agents may affect the growth of Campylobacter jejuni?

Polymyxin

None of these

Trimethoprim

Vacomycin

TRUE ANSWER : ?
YOUR ANSWER : ?

Plate counts are often reported as

cfu

thousands/ml

cfu/ml

Both (a) and (b)

TRUE ANSWER : ?
YOUR ANSWER : ?

Viable plate count can be obtained using

Pour plate method

Hemocytometer

Spread plate method

both (a) and (b)

TRUE ANSWER : ?
YOUR ANSWER : ?

The solidifying agent commonly used in preparation of media is/are

agar

none of these

both (a) and (b)

silica gel

TRUE ANSWER : ?
YOUR ANSWER : ?

Cell counting can be carried out by

membrane filter count

All of these

plate counting

direct microscopic count using petroffhansser counting chamber

TRUE ANSWER : ?
YOUR ANSWER : ?

Stuart's transport medium is used for transport of specimen containing

Salmonella

Vibrio cholerae

Neisseria gonorrhoeae

Shigella

TRUE ANSWER : ?
YOUR ANSWER : ?

For the selection of endospore-forming bacteria, a mixed culture can be heated at

70°C for 10 minutes

80°C for 10 minutes

90°C for 10 minutes

60°C for 10 minutes

TRUE ANSWER : ?
YOUR ANSWER : ?

Which of the following problem makes it impossible to satisfy all of Koch's postulates?

Genes from the microorganism cannot be amplified by PCR

Microorganism cannot be isolated in pure culture

Microorganism causes serious symptoms in humans

Species of microorganism is in question

TRUE ANSWER : ?
YOUR ANSWER : ?

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