BRUCELLA QUIZ

Proofreading activity to maintain the fidelity of DNA synthesis

occurs after the synthesis has been completed

occurs in prokaryotes but not eukaryotes

is a function of the 3'-5' exonuclease activity of the DNA polymerases

requires the presence of an enzyme separate from the DNA polymerases

TRUE ANSWER : ?
YOUR ANSWER : ?

During which of the following process a new copy of a DNA molecule is precisely synthesized?

Trasformation

Transcription

Translation

Replication

TRUE ANSWER : ?
YOUR ANSWER : ?

Which of the following statements on replication in E.coli is correct?

Occurs only when λ phase has infected E. coli

It occurs in bidirectional manner

Always uses T7 DNA polymerase when infected by T7 phage

It occurs in a unidirectional manner

TRUE ANSWER : ?
YOUR ANSWER : ?

Both strands of DNA serve as templates concurrently in

none of these

excision repair

replication

mismatch repair

TRUE ANSWER : ?
YOUR ANSWER : ?

The enzyme that catalyzes the synthesis of DNA is called

DNA ligase

DNA polymerase

DNA gyrase

helicase

TRUE ANSWER : ?
YOUR ANSWER : ?

DNA gyrase is inhibited by

cephalosporin

both (a) and (b)

nalidixic acid

tetracycline

TRUE ANSWER : ?
YOUR ANSWER : ?

Which of the following possesses both 5'-3' and 3'-5' exonuclease activity?

Kornberg enzyme

Taq DNA polymerase

DNA polymerase III

None of these

TRUE ANSWER : ?
YOUR ANSWER : ?

During DNA replication in prokaryotes, synthesis begins on the circular chromosome

always at the same place

at the promoter

randomly on the chromosome

at any stretch of DNA that is high in AT pairs

TRUE ANSWER : ?
YOUR ANSWER : ?

In E.coli, which enzyme synthesizes the RNA primer for Okazaki fragments?

DnaG

all of these

DnaA

DnaC

TRUE ANSWER : ?
YOUR ANSWER : ?

Which of the following enzymes unwind short stretches of DNA helix immediately ahead of a replication fork?

DNA polymerases

Helicases

Topoisomerases

Single-stranded binding proteins

TRUE ANSWER : ?
YOUR ANSWER : ?

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