BIOSENSORS AND BIOCHIPS QUIZ

Size of the DNA that can be packaged into a λ phage is

any size

40-50 kb

35-53 kb

50 kb

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λ gt 10 and λ gt 11 vectors can propagate cloned fragments up to

6-7 kb

20-23 kb

40-44 kb

1-2 kb

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P1 cloning vector allow cloning of DNA of the length of

50 kbp

100 kbp

10 kbp

20 kbp

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Phagemid consist of

plasmid vector carrying λ phage's cos site

plasmid vector carrying origin of replication of λ phage only

plasmid vector carrying λ attachment (λ att) site

plasmid vector carrying origin of replication of plasmid only

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Charon 34 and Charon 35 can clone DNA upto

6-7 kb

9-20 kb

30-35 kb

1-2 kb

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EMBL 3 and EMBL 4 are replacement vectors, which can clone DNA up to

6-7 kb

15-25 kb

40-44 kb

1-2 kb

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Single stranded vectors are useful

for oligonucleotide directed mutagenesis

for sequencing of cloned DNA

All of these

for probe preparation

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Cosmid vectors are used for

cloning prokaryotic DNA only

cloning large fragments of DNA

cloning eukaryotic DNA only

cloning small fragments of DNA

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Phagemid vectors are

phages carrying properties of plasmids

combination of phages and cosmid

All of these

combination of plasmid and phage λ

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Inserted DNA in λ gt 11 can be expressed as

free protein that is secreted out

β-galactosidase fused protein

free protein in the cytoplasm

All of these

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