BIOSENSORS AND BIOCHIPS QUIZ

Which of the following repairs nicked DNA by forming a phosphodiester bond between adjacent nucleotides?

DNA gyrase

DNA ligase

Topoisomerases

Helicase

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The replication of chromosomes by eukaryotes occurs in a relatively short period of time because

the eukaryotes have more amount of DNA for replication

each chromosome contains multiple replicons

the eukaryotic replication machinery is 1000 times faster than the prokaryotes

eukaryotic DNA is always single stranded

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Both strands of DNA serve as templates concurrently in

excision repair

none of these

replication

mismatch repair

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Which of the following enzymes unwind short stretches of DNA helix immediately ahead of a replication fork?

Single-stranded binding proteins

Helicases

DNA polymerases

Topoisomerases

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Which of the following possesses both 5'-3' and 3'-5' exonuclease activity?

DNA polymerase III

None of these

Kornberg enzyme

Taq DNA polymerase

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During which of the following process a new copy of a DNA molecule is precisely synthesized?

Translation

Transcription

Replication

Trasformation

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The enzyme that catalyzes the synthesis of DNA is called

DNA gyrase

DNA ligase

helicase

DNA polymerase

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DNA gyrase is inhibited by

tetracycline

cephalosporin

both (a) and (b)

nalidixic acid

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Which of the following lacks 3'-5' exonuclease activity?

All of these

Taq DNA polymerase

Short fragment of DNA polymerase I

T4 DNA ligase

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Proofreading activity to maintain the fidelity of DNA synthesis

is a function of the 3'-5' exonuclease activity of the DNA polymerases

occurs after the synthesis has been completed

occurs in prokaryotes but not eukaryotes

requires the presence of an enzyme separate from the DNA polymerases

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