Very high sugar concentration (40-100 g/l) have been used none of these to adjust the osmotic potential of the media in short term treatment for regeneration in specialized secondary metabolite production Both (a) and (b) TRUE ANSWER : ? YOUR ANSWER : ?
Which of the following is not true about nurse or conditioned medium? It contains uncharacterized growth factor released by growing cells it is removed aseptically from the culture and is autoclaved before use It is liquid removed from the suspension of fast growing cells It is used in the culture of regenerating protoplast TRUE ANSWER : ? YOUR ANSWER : ?
Which of the following growth regulator is added for short initiation during plant regeneration from callus? Cytokinins Gibberellins Auxins Brassinosteroids TRUE ANSWER : ? YOUR ANSWER : ?
In plant cell culture media, auxins and cytokinins are used in the range of l-50μM more than 125μM 50-100μM 100-125μM TRUE ANSWER : ? YOUR ANSWER : ?
Concentration of sucrose generally used in plant cell culture media is 60-70 g/l 40-50 g/l 20-30 g/l 10-15 g/l TRUE ANSWER : ? YOUR ANSWER : ?
Which is/are the disadvantage/(s) of using IAA in plant cell culture media? It is unstable in solution Gets easily oxidized All of these Conjugated to inactive form by plant cells TRUE ANSWER : ? YOUR ANSWER : ?
Neutralized activated charcoal is occasionally added to young regenerating cultures to help to remove plants growth regulators introduced at an earlier stage remove toxic phenolics produced by the stressed plant cell both (a) and (b) maintain the pH of the medium TRUE ANSWER : ? YOUR ANSWER : ?
Silver thiosulphate is added to culture medium as it helps to remove toxic phenolics from plant cells prevent the gaseous plant hormone, ethylene dioxide from accumulating to detrimental condition. All of these maintain the pH TRUE ANSWER : ? YOUR ANSWER : ?
Nitrogen in the plant cell culture media is provided by either ammonia or nitrate salt. In the media utilization of both ammonium and nitrate result in rise in pH utilization of ammonium cause culture pH to drop while utilization of nitrate cause culture pH to rise utilization of nitrate cause culture pH to drop while utilization of ammonium cause culture pH to rise utilization of both ammonium and nitrate result in drop in pH TRUE ANSWER : ? YOUR ANSWER : ?
Which is/are the naturally occurring plant auxins? Indole acetic acid (IAA) 2,4-dichlorophenoxyacetic acid Naphthalenacetic acid (NAA) All of these TRUE ANSWER : ? YOUR ANSWER : ?