Higher dissolved oxygen concentration in the culture media are toxic and lead to All of these lipid peroxidation DNA degradation metabolism of nutrients in culture media at a rate greater than that required for consumption TRUE ANSWER : ? YOUR ANSWER : ?
Sometimes cell lines can be cultured for such a long time that they apparently develop the potential to be subcultured indefinitely in vitro. Such cells lines are called established cell lines primary cell lines propagated cell lines secondary cell lines TRUE ANSWER : ? YOUR ANSWER : ?
According to Eagle, the growth of L-strain and Hela-strain cultures require to have mandatory presence of 6 amino acids 8 amino acids 10 amino acids 12 amino acids TRUE ANSWER : ? YOUR ANSWER : ?
Toxicity due to accumulation of ammonia can be overcome by removal of ammonia or ammonium from culture medium by controlled addition of glutamine at low level All of these by substituting glutamine by glutamate TRUE ANSWER : ? YOUR ANSWER : ?
Cells which have undergone transformation frequently become anchorage independent anchorage dependent stable unstable TRUE ANSWER : ? YOUR ANSWER : ?
Range of optimum glutamine concentration present in the culture media is 1-2 mmol/litre 2-7 mmol/litre 7-15 mmol/litre 15 - 20 mmol/litre TRUE ANSWER : ? YOUR ANSWER : ?
During the growth of animal cells in culture, it is noticed that the cells do not look very healthy. After an investigation, this is found that there is a lot of lactic acid in the culture fluid. What is probably wrong with this culture? The cells do not have enough oxygen Glycolysis is being inhibited The cells have too much oxygen Ethyl alcohol is being produced in excess TRUE ANSWER : ? YOUR ANSWER : ?
The human fibroplast is a classical example of cell transformations none of these stable primary cell lines established cell lines TRUE ANSWER : ? YOUR ANSWER : ?
When dissolved oxygen is lower than the critical concentration, viable cell concentration declines because of both (a) and (b) accumulation of ammonia increase in specific lactate production from glucose incomplete glutamine oxidation TRUE ANSWER : ? YOUR ANSWER : ?
For culturing, plasma from the adult chicken is preferred to mammalian plasma because it forms a semi solid coagulum it is too opaque it forms a clear and solid coagulum even after dilution it doesn't produce solid clots TRUE ANSWER : ? YOUR ANSWER : ?