ANIMAL CELL CULTURE AND REGULATION QUIZ

Lactate and ammonium

Lactate only

CO2 and NH3

CO2 and lactate

reduction in the pH of culture hence loss of cell viability

increase in pH

no loss of cell viability

no change in pH

by removal of ammonia or ammonium from culture medium

by controlled addition of glutamine at low level

All of these

by substituting glutamine by glutamate

uptake of new genetic material

both (a) and (b)

release of genetic information

phenotypic modifications of cells in culture

accumulation of ammonia

both (a) and (b)

incomplete glutamine oxidation

increase in specific lactate production from glucose

established cell lines

none of these

cell transformations

stable primary cell lines

DNA degradation

All of these

lipid peroxidation

metabolism of nutrients in culture media at a rate greater than that required for consumption

All of these

Organ cultures on plasma clots

Whole embryo cultures

Organ cultures on agar

secondary cell lines

primary cell lines

propagated cell lines

established cell lines

5.5 - 55 mmol/litre

105-150 mmol/litre

75-105 mmol/litre

55 - 75 mmol/litre