What are the main constituents of culture for animal cell growth? Cytokines All of these Glucose and Glutamine Growth factors TRUE ANSWER : ? YOUR ANSWER : ?
The major problem associated with the isolation of free cells and cell aggregates from organs is that of releasing the cells from their supporting matrix inhibiting the cells from their supporting matrix disintegrating the cells from their supporting matrix None of these TRUE ANSWER : ? YOUR ANSWER : ?
Accumulation of lactate leads to no change in pH no loss of cell viability increase in pH reduction in the pH of culture hence loss of cell viability TRUE ANSWER : ? YOUR ANSWER : ?
Specific oxygen consumption rates for mammalian cells are in the range of (where n and p represents to nano and pico respectively) 5 - 10 pmol of O2/cell/h 0.05-5 nmol of O2/cell/h 0.05-5 pmol of O2/cell/h 5 - 10 nmol of O2 /cell/h TRUE ANSWER : ? YOUR ANSWER : ?
During the growth of animal cells in culture, it is noticed that the cells do not look very healthy. After an investigation, this is found that there is a lot of lactic acid in the culture fluid. What is probably wrong with this culture? The cells do not have enough oxygen Glycolysis is being inhibited Ethyl alcohol is being produced in excess The cells have too much oxygen TRUE ANSWER : ? YOUR ANSWER : ?
What is the concentration of CO2 required for culturing animal cells? 10-15% 15-20% 1-10% 2-5% TRUE ANSWER : ? YOUR ANSWER : ?
Cells which have undergone transformation frequently become anchorage dependent unstable stable anchorage independent TRUE ANSWER : ? YOUR ANSWER : ?
pH of culture medium is initially controlled by addition of bases presence of CO2 presence of bicarbonate buffer none of these TRUE ANSWER : ? YOUR ANSWER : ?
According to Eagle, the growth of L-strain and Hela-strain cultures require to have mandatory presence of 6 amino acids 10 amino acids 12 amino acids 8 amino acids TRUE ANSWER : ? YOUR ANSWER : ?
Range of osmolarity tolerated/accepted in mOsm/Kg of H2O by mammalian cells is 280-360 300-325 360-400 150-300 TRUE ANSWER : ? YOUR ANSWER : ?