ANIMAL CELL CULTURE AND REGULATION QUIZ

anchorage independent

stable

anchorage dependent

unstable

established cell lines

secondary cell lines

propagated cell lines

primary cell lines

phenotypic modifications of cells in culture

release of genetic information

both (a) and (b)

uptake of new genetic material

15-20%

10-15%

1-10%

2-5%

Glycolysis is being inhibited

The cells do not have enough oxygen

The cells have too much oxygen

Ethyl alcohol is being produced in excess

15 - 20 mmol/litre

2-7 mmol/litre

1-2 mmol/litre

7-15 mmol/litre

Helps in the cells respiration

It participates in the de novo synthesis of purines and pyrimidines

All of these

For monitoring pH of the culture

0.05-5 nmol of O2/cell/h

5 - 10 nmol of O2 /cell/h

0.05-5 pmol of O2/cell/h

5 - 10 pmol of O2/cell/h

Lactate only

CO2 and lactate

Lactate and ammonium

CO2 and NH3

established cell lines

stable primary cell lines

cell transformations

none of these