What is the concentration of CO2 required for culturing animal cells? 2-5% 15-20% 1-10% 10-15% TRUE ANSWER : ? YOUR ANSWER : ?
Excess CO2 suppress cell growth and productivity by altering pH of the medium altering intracellular pH by diffusing across cell membrane inhibiting respiration both (a) and (b) TRUE ANSWER : ? YOUR ANSWER : ?
Which of the following abnormality, resulted from the inheritance of an unbalanced complement of chromosomes can be diagnosed through karotyping? Klinefelter's syndrome Down's syndrome Turner's syndrome all of these TRUE ANSWER : ? YOUR ANSWER : ?
An established cell line can be called where it has been subcultured at least 70 times at an interval of 3 days between subcultures 40 times at an interval of 3 days between subcultures 70 times at an interval of 1 day between subcultures 50 times at an interval of 3 days between subcultures TRUE ANSWER : ? YOUR ANSWER : ?
pH of culture medium is initially controlled by presence of CO2 presence of bicarbonate buffer addition of bases none of these TRUE ANSWER : ? YOUR ANSWER : ?
What is the effect of excess accumulation of metabolite products (lactate and ammonium) on cells? They act as growth promoters Lactate helps in the growth while ammonium inhibits the growth Have no effect on cells They act as growth inhibitors TRUE ANSWER : ? YOUR ANSWER : ?
To prevent the accumulation of lactate high glutamine concentration is required low glucose concentration is required high glucose concentration is required low glutamine concentration is required TRUE ANSWER : ? YOUR ANSWER : ?
Sometimes cell lines can be cultured for such a long time that they apparently develop the potential to be subcultured indefinitely in vitro. Such cells lines are called established cell lines primary cell lines secondary cell lines propagated cell lines TRUE ANSWER : ? YOUR ANSWER : ?
Range of optimum glutamine concentration present in the culture media is 15 - 20 mmol/litre 1-2 mmol/litre 7-15 mmol/litre 2-7 mmol/litre TRUE ANSWER : ? YOUR ANSWER : ?
During the growth of animal cells in culture, it is noticed that the cells do not look very healthy. After an investigation, this is found that there is a lot of lactic acid in the culture fluid. What is probably wrong with this culture? The cells have too much oxygen Ethyl alcohol is being produced in excess The cells do not have enough oxygen Glycolysis is being inhibited TRUE ANSWER : ? YOUR ANSWER : ?