What is the effect of excess accumulation of metabolite products (lactate and ammonium) on cells? They act as growth inhibitors Lactate helps in the growth while ammonium inhibits the growth Have no effect on cells They act as growth promoters TRUE ANSWER : ? YOUR ANSWER : ?
Which of the followings are the metabolic products of glucose and glutamine? Lactate only Lactate and ammonium CO2 and NH3 CO2 and lactate TRUE ANSWER : ? YOUR ANSWER : ?
How does CO2 help in the cell metabolism during cell culture? All of these For monitoring pH of the culture It participates in the de novo synthesis of purines and pyrimidines Helps in the cells respiration TRUE ANSWER : ? YOUR ANSWER : ?
Sometimes cell lines can be cultured for such a long time that they apparently develop the potential to be subcultured indefinitely in vitro. Such cells lines are called primary cell lines propagated cell lines secondary cell lines established cell lines TRUE ANSWER : ? YOUR ANSWER : ?
Range of optimum glutamine concentration present in the culture media is 1-2 mmol/litre 15 - 20 mmol/litre 2-7 mmol/litre 7-15 mmol/litre TRUE ANSWER : ? YOUR ANSWER : ?
Cells which have undergone transformation frequently become anchorage independent unstable anchorage dependent stable TRUE ANSWER : ? YOUR ANSWER : ?
Which of the following abnormality, resulted from the inheritance of an unbalanced complement of chromosomes can be diagnosed through karotyping? all of these Turner's syndrome Down's syndrome Klinefelter's syndrome TRUE ANSWER : ? YOUR ANSWER : ?
What is the concentration of CO2 required for culturing animal cells? 1-10% 10-15% 2-5% 15-20% TRUE ANSWER : ? YOUR ANSWER : ?
According to Eagle, the growth of L-strain and Hela-strain cultures require to have mandatory presence of 6 amino acids 10 amino acids 8 amino acids 12 amino acids TRUE ANSWER : ? YOUR ANSWER : ?
Toxicity due to accumulation of ammonia can be overcome by substituting glutamine by glutamate All of these by removal of ammonia or ammonium from culture medium by controlled addition of glutamine at low level TRUE ANSWER : ? YOUR ANSWER : ?