ANIMAL CELL CULTURE AND REGULATION QUIZ

enzymatic digestion

All of these

treating with chelating agents

physical disruption

altering intracellular pH by diffusing across cell membrane

altering pH of the medium

both (a) and (b)

inhibiting respiration

it forms a semi solid coagulum

it forms a clear and solid coagulum even after dilution

it is too opaque

it doesn't produce solid clots

increase in specific lactate production from glucose

accumulation of ammonia

incomplete glutamine oxidation

both (a) and (b)

All of these

by removal of ammonia or ammonium from culture medium

by controlled addition of glutamine at low level

by substituting glutamine by glutamate

both (a) and (b)

uptake of new genetic material

release of genetic information

phenotypic modifications of cells in culture

solid medium

liquid medium

both (a) and (b)

semi-solid medium

0.05-5 nmol of O2/cell/h

5 - 10 nmol of O2 /cell/h

0.05-5 pmol of O2/cell/h

5 - 10 pmol of O2/cell/h

none of these

presence of bicarbonate buffer

addition of bases

presence of CO2

15-20%

1-10%

2-5%

10-15%