Range of osmolarity tolerated/accepted in mOsm/Kg of H2O by mammalian cells is 360-400 300-325 150-300 280-360 TRUE ANSWER : ? YOUR ANSWER : ?
What is the concentration of CO2 required for culturing animal cells? 10-15% 15-20% 1-10% 2-5% TRUE ANSWER : ? YOUR ANSWER : ?
When dissolved oxygen is lower than the critical concentration, viable cell concentration declines because of incomplete glutamine oxidation accumulation of ammonia both (a) and (b) increase in specific lactate production from glucose TRUE ANSWER : ? YOUR ANSWER : ?
Which of the followings are the metabolic products of glucose and glutamine? Lactate only CO2 and NH3 CO2 and lactate Lactate and ammonium TRUE ANSWER : ? YOUR ANSWER : ?
The technique of organ culture may be divided on the basis of employing semi-solid medium both (a) and (b) liquid medium solid medium TRUE ANSWER : ? YOUR ANSWER : ?
How does CO2 help in the cell metabolism during cell culture? Helps in the cells respiration All of these It participates in the de novo synthesis of purines and pyrimidines For monitoring pH of the culture TRUE ANSWER : ? YOUR ANSWER : ?
Accumulation of lactate leads to no change in pH no loss of cell viability increase in pH reduction in the pH of culture hence loss of cell viability TRUE ANSWER : ? YOUR ANSWER : ?
What are the main constituents of culture for animal cell growth? All of these Cytokines Glucose and Glutamine Growth factors TRUE ANSWER : ? YOUR ANSWER : ?
During the growth of animal cells in culture, it is noticed that the cells do not look very healthy. After an investigation, this is found that there is a lot of lactic acid in the culture fluid. What is probably wrong with this culture? Glycolysis is being inhibited The cells have too much oxygen Ethyl alcohol is being produced in excess The cells do not have enough oxygen TRUE ANSWER : ? YOUR ANSWER : ?
Which of the following is not a source of energy in active muscle cells? Creatine phosphate Lactic acid Glucose ATP TRUE ANSWER : ? YOUR ANSWER : ?