ANIMAL CELL CULTURE AND REGULATION QUIZ

2-7 mmol/litre

15 - 20 mmol/litre

1-2 mmol/litre

7-15 mmol/litre

0.05-5 nmol of O2/cell/h

5 - 10 nmol of O2 /cell/h

5 - 10 pmol of O2/cell/h

0.05-5 pmol of O2/cell/h

8 amino acids

12 amino acids

10 amino acids

6 amino acids

They act as growth promoters

Have no effect on cells

They act as growth inhibitors

Lactate helps in the growth while ammonium inhibits the growth

cell transformations

established cell lines

none of these

stable primary cell lines

Creatine phosphate

ATP

Glucose

Lactic acid

inhibiting the cells from their supporting matrix

None of these

disintegrating the cells from their supporting matrix

releasing the cells from their supporting matrix

Whole embryo cultures

Organ cultures on plasma clots

All of these

Organ cultures on agar

150-300

360-400

280-360

300-325

All of these

by substituting glutamine by glutamate

by controlled addition of glutamine at low level

by removal of ammonia or ammonium from culture medium