ALLOSTERIC EFFECTS QUIZ

movement of the F-helix, which contains the proximal His

binding of oxygen to the heme

movement of the proximal histidine towards the heme

reorganization of protein-protein contacts between the individual subunits

only be found in either the unliganded form or the fully liganded form

show a Hill coefficient (nH) of 0.0

show a Hill coefficient (nH) of n

both (b) and (c)

BPG binds to the R state with the same affinity as the T state

it is displaced from the heme by oxygen

its binding pocket becomes too small to accommodate BPG

it is displaced from the heme by movement of the proximal histidine

to minimize oxygen delivery to the tissues

to maximize oxygen delivery to the tissues

for maintaining Fe in the Fe2+ state

only in humans

4.5 and 1.2

1.2 and 4.5

1.0 and 2.8

2.8 and 1.0

a hyperbolic binding curve

both (b) and (c)

a linear Scatchard Plot

a sigmodial binding curve

the amino acid residues lining the binding site

the absence of competing ligands

the presence of hydrating water molecules

the opposite chirality of the binding ligand

1

None of these

not defined

2

stabilizes the R state of the protein

decreases the binding affinity

increases the binding affinity

both (a) and (c)

100-fold higher affinity for the last O2 bound than for the first

extensive protein conformational change

both (a) and (b)

100-fold lower affinity for the last O2 bound than for the first