ALLOSTERIC EFFECTS QUIZ

the absence of competing ligands

the amino acid residues lining the binding site

the presence of hydrating water molecules

the opposite chirality of the binding ligand

2

None of these

not defined

1

show a Hill coefficient (nH) of 0.0

only be found in either the unliganded form or the fully liganded form

both (b) and (c)

show a Hill coefficient (nH) of n

to maximize oxygen delivery to the tissues

to minimize oxygen delivery to the tissues

only in humans

for maintaining Fe in the Fe2+ state

a linear Scatchard Plot

both (b) and (c)

a sigmodial binding curve

a hyperbolic binding curve

reorganization of protein-protein contacts between the individual subunits

movement of the F-helix, which contains the proximal His

movement of the proximal histidine towards the heme

binding of oxygen to the heme

1.0 and 2.8

2.8 and 1.0

4.5 and 1.2

1.2 and 4.5

BPG binds to the R state with the same affinity as the T state

it is displaced from the heme by movement of the proximal histidine

it is displaced from the heme by oxygen

its binding pocket becomes too small to accommodate BPG

100-fold higher affinity for the last O2 bound than for the first

both (a) and (b)

100-fold lower affinity for the last O2 bound than for the first

extensive protein conformational change

increases the binding affinity

stabilizes the R state of the protein

decreases the binding affinity

both (a) and (c)