A plasmid always contains an origin of replication is a circular DNA molecule usually contains one or more restriction sites All of these TRUE ANSWER : ? YOUR ANSWER : ?
Which of the following is correct in terms of determination of location of genetic traits? Protein-coding genes are always associated with a restriction pattern Restriction sites allow DNAs to be digested None of these Known protein coding sequences are too far apart to allow linkage determination for most new genes TRUE ANSWER : ? YOUR ANSWER : ?
The order for the construction of a cDNA fragment from mRNA is to treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase and bind oligo-dT digest with RNase, add G residues to the 3' end, treat with reverse transcriptase, add G residues to the 3' end and treat with DNA polymerase bind oligo-dC, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dT and treat with DNA polymerase bind oligo-dT, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase TRUE ANSWER : ? YOUR ANSWER : ?
Which of these restriction enzymes produce blunt ends? HindIII EcoRV SaII XhoI TRUE ANSWER : ? YOUR ANSWER : ?
Which of the following is not true of small inbreeding populations? Mutation is increased Alleles may become fixed There is an increased incidence of recessive diseases They tend to loose genetic diversity TRUE ANSWER : ? YOUR ANSWER : ?
In gel electrophoresis, DNA molecules migrate from __________ to __________ ends of the gel. long ... short negative ... positive positive to negative basic ... acidic TRUE ANSWER : ? YOUR ANSWER : ?
Restriction enzymes are named for the viral DNA that they attack the bacterium they are derived from None of these the person who discovered TRUE ANSWER : ? YOUR ANSWER : ?
Which type of restriction enzymes do not usually require ATP? Type I Type II Type IV Type III TRUE ANSWER : ? YOUR ANSWER : ?
First discovered, Type II restriction endonuclease was Hinf I EcoRI Hind II Eco K TRUE ANSWER : ? YOUR ANSWER : ?
In order to insert a foreign gene into a plasmid, both must __________ have identical DNA sequences be cut by the same restriction enzyme originate from the same type of cell be of the same length TRUE ANSWER : ? YOUR ANSWER : ?