Microbial Recombination and Gene Transfer
The expression of gene X (which has promoter Px) is to be monitored. A gene fusion construction for carrying this work will

have the promoter of lacZ or some other reporter gene
give the same information as from a microarray
have Px but not the rest of the X coding region
allow to monitor the expression of all genes with a promoter similar in sequence to Px

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Microbial Recombination and Gene Transfer
The main difference between a self-transmissible and a mobilizable plasmid is that the self-transmissible plasmid

transfers antibiotic resistance genes
transfers both strands of the plasmid DNA
carries genes encoding the mating apparatus
usually has a transposon inserted into it

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