Microbial Genetics
In the time since E. coli and Salmonella diverged evolutionarily

E, coli has lost approximately 50% of its genome
none of these
there has been little change in either genome
E. coli has acquired many genes via horizontal transfer

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Microbial Genetics
Why the bacterium Treponema pallidum is difficult to culture?

Because it requires extremely low temperature at which water freezes
Because it requires a great deal of water to reproduce
Because it lacks the genes needed for TCA cycle and oxidative phosphorylation
Because it is unable to use carbohydrates as an energy source

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