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Cell Cultures and Characteristics

Cell Cultures and Characteristics
In the pour plate method, the mixed culture is diluted directly in tubes of

both (a) and (b)
liquid agar medium
sterile liquid usually water
none of these

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Cell Cultures and Characteristics
To select cholera-causing bacterium, Vibrio cholerae, the pH of the medium is maintained at a pH of

3.5
8.90
6.79
8.5

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Cell Cultures and Characteristics
Cell counting can be carried out by

membrane filter count
All of these
direct microscopic count using petroffhansser counting chamber
plate counting

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Cell Cultures and Characteristics
Agar is used for solidifying culture media because

the melting and solidifying points of agar solution are not the same
it does not add to the nutritive properties of the medium
All of these
it does not affect by the growth of bacteria

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Cell Cultures and Characteristics
Which of the following is used for the proper maintenance and preservation of pure cultures?

Preservation by overlaying cultures with mineral oil
All of these
Preservation by lyophilization
Periodic transfer to fresh media

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Cell Cultures and Characteristics
Selective media facilitate growth of only one kind of organism. Saboraud medium is used to selectively isolate

coliform bacteria
yeasts
gram positive bacteria
acid fast organisms

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