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Cell Cultures and Characteristics

Cell Cultures and Characteristics
In the pour plate method, the mixed culture is diluted directly in tubes of

none of these
sterile liquid usually water
liquid agar medium
both (a) and (b)

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Cell Cultures and Characteristics
To select cholera-causing bacterium, Vibrio cholerae, the pH of the medium is maintained at a pH of

8.90
8.5
3.5
6.79

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Cell Cultures and Characteristics
Which of the following methods is most likely to be quantitative?

None of these
Dilution and plating
Wet mount
Gram staining

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Cell Cultures and Characteristics
In the process of freeze drying, a dense cell suspension is placed in small vials and is frozen at

-30 to -48°C
-20 to -30°C
-60 to -78°C
-48 to -58°C

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Cell Cultures and Characteristics
Which of the following is used for the proper maintenance and preservation of pure cultures?

Preservation by lyophilization
Preservation by overlaying cultures with mineral oil
Periodic transfer to fresh media
All of these

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Cell Cultures and Characteristics
The solidifying agent commonly used in preparation of media is/are

both (a) and (b)
silica gel
none of these
agar

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