Glycolysis
In glycolysis, ATP is formed by the transfer of a high-energy phosphate from 1,3-bisphosphoglycerate to ADR No such high-energy phosphate donor has ever been isolated in mitochondria because

no such phosphate donor exists
the high-energy phosphate donor is very short-lived and difficult to isolate
None of these
the techniques for isolating the phosphate donor are not refined enough

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Glycolysis
Which of the following is not a mechanism for altering the flux of metabolites through the rate-determining step of a pathway?

Diffusional coupling between adjacent active sites
Allosteric control of the enzyme activity
Covalent modification of the enzyme
Genetic control of the enzyme concentration

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Glycolysis
A kinase is an enzyme that

uses NADH to change the oxidation state of the substrate
removes water from a double bond
removes phosphate groups of substrates
uses ATP to add a phosphate group to the substrate

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Glycolysis
Why does the glycolytic pathway continue in the direction of glucose catabolism?

Glycolysis occurs in either direction
High levels of ATP keep the pathway going in a forward direction
There are essentially three irreversible reactions that act as the driving force for the pathway
The enzymes of glycolysis only function in one direction

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