Glycolysis
In glycolysis, ATP is formed by the transfer of a high-energy phosphate from 1,3-bisphosphoglycerate to ADR No such high-energy phosphate donor has ever been isolated in mitochondria because

the techniques for isolating the phosphate donor are not refined enough
the high-energy phosphate donor is very short-lived and difficult to isolate
None of these
no such phosphate donor exists

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Glycolysis
Which of the following is not a mechanism for altering the flux of metabolites through the rate-determining step of a pathway?

Allosteric control of the enzyme activity
Covalent modification of the enzyme
Diffusional coupling between adjacent active sites
Genetic control of the enzyme concentration

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Glycolysis
Which of the following is not true of glycolysis?

The pathway requires two moles of ATP to get started catabo-lizing each mole of glucose
ADP is phosphorylated to ATP via substrate level phosphorylation
The pathway does not require oxygen
The pathway oxidizes two moles of NADH to NAD+ for each mole of glucose that enters

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Glycolysis
A kinase is an enzyme that

removes water from a double bond
uses ATP to add a phosphate group to the substrate
removes phosphate groups of substrates
uses NADH to change the oxidation state of the substrate

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