Polymerase Chain Reaction in Biochemistry
A PCR cycle consists of

three steps, initiation, elongation and termination
three steps, denaturation, initiation and elongation
three steps, denaturation, primer annealing and elongation
three steps, primer annealing, elongation and termination

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Polymerase Chain Reaction in Biochemistry
To clone into a plasmid vector, both the plasmid and the foreign DNA are cut

with the combination of enzymes and then seperated
with the same restriction enzyme and mixed together
with different restriction enzyme and mixed together
with the combination of enzymes and mixed together

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Polymerase Chain Reaction in Biochemistry
Southern blotting involves

electrophoresis of DNA molecules and then blotting the separated RNA bands followed by incubation
electrophoresis of DNA molecules and then blotting the separated DNA bands followed by incubation
electrophoresis of DNA molecules followed by incubation
electrophoresis of RNA molecules and then blotting the separated RNA bands followed by incubation

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