Glycolysis
Which of the following is not true of glycolysis?

The pathway oxidizes two moles of NADH to NAD+ for each mole of glucose that enters
ADP is phosphorylated to ATP via substrate level phosphorylation
The pathway does not require oxygen
The pathway requires two moles of ATP to get started catabo-lizing each mole of glucose

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Glycolysis
In glycolysis, ATP is formed by the transfer of a high-energy phosphate from 1,3-bisphosphoglycerate to ADR No such high-energy phosphate donor has ever been isolated in mitochondria because

the high-energy phosphate donor is very short-lived and difficult to isolate
None of these
the techniques for isolating the phosphate donor are not refined enough
no such phosphate donor exists

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