Recombinant DNA
Enzymes that recognize and cleave specific 4 to 8 base pair sequences of DNA are

restriction endonucleases
helicases
DNA gyrase
DNA ligase

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Recombinant DNA
The advantage of using DNA polymerases from thermophilic organisms in PCR is that

the DNA polymerases of these bacteria never make mistakes while replicating DNA
the DNA polymerases of these bacteria are much faster than those from other organisms
All of these
the DNA polymerases of these bacteria can withstand the high temperatures needed to denature the DNA strands

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Recombinant DNA
Bacterial cells protect their own DNA from degradation by restriction endonucleases by

deleting all recognition sites from the genome
methylating the DNA at the sites that the enzyme recognizes
having anti restriction endonucleases
not producing any restriction endonucleases

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