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Gene Transfer in Plants

Gene Transfer in Plants
Direct DNA uptake by protoplasts can be stimulated by

decanal
luciferin
polyethylene glycol (PEG)
all of these

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Gene Transfer in Plants
Because of large size of Ti-plasmid, intermediate vectors (IV) are developed in which T DNA has been subcloned into

pRK 2013
pRN 3
all of these
pBR 322 based plasmid vector

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Gene Transfer in Plants
In the liposome mediated gene transfer in plants, nucleic acids are

both (a) and (b)
stable in liposomes
protected from nuclease digestion
not stable in liposomes

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Gene Transfer in Plants
Which of the following genes are constitutively expressed and control the plant induced activation of other vir genes?

vir C and vir D
vir B and vir E
vir A and vir B
vir A and vir G

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Gene Transfer in Plants
Which of the following is not true about the direct repeats flanking T-DNA?

These repeats are transferred intact to the plant genome
All of these
They are conserved between nopaline and octopine Ti-plasmids
These are important in integration mechanism

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Gene Transfer in Plants
Vir genes required for the T-DNA transfer and processing are located

outside the T-DNA region
on the plant genome
none of these
on the T-DNA

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