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Gene Transfer in Plants

Gene Transfer in Plants
Direct DNA uptake by protoplasts can be stimulated by

decanal
polyethylene glycol (PEG)
luciferin
all of these

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Gene Transfer in Plants
The transfer of intermediate vectors into Agrobacterium are brought about by

transformation
biparental mating
triparental mating
transduction

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Gene Transfer in Plants
Intermediate vectors containing T-DNA are conjugation deficient. Thus conjugation is mediated in presence of which of the following plasmid?

either (a) or (b)
pRN 3
pRK 2013
none of these

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Gene Transfer in Plants
In response to the activating signal molecule, an endonuclease is produced that causes nicks in the T-DNA. It is encoded by

vir A
vir B
vir D
vir C

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Gene Transfer in Plants
Microinjection involves

injection with needle having diameter greater than cell diameter
injection of DNA into bigger cells
All of these
injection of large amount of DNA

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Gene Transfer in Plants
The size of the virulent plasmid of Agrobacterium tumefaciens is

40-80 kb
140-235 kb
80-120 kb
>235 kb

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