DNA Structure and Replication
Automated DNA sequencing uses the chain termination method

either (b) or (c)
but with an oligonucleotide primer labeled with a fluorescent dye
with no primer
but with a polynucleotide primer labeled with a fluorescent dye

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DNA Structure and Replication
In DNA, nucleotides are covalently joined together by

3', 5' phosphodiester bonds to form a repetitive sugar-phosphate chain
2', 3' phosphodiester bonds to form a repetitive sugar-phosphate chain
2', 5' phosphodiester bonds to form a repetitive sugar-phosphate chain
3', 4' phosphodiester bonds to form a repetitive sugar-phosphate chain

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