HPLC
An isocratic elution in HPLC is one in which the composition of the solvent

remains constant
changes in a series of steps
none of these
changes continuously

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HPLC
Column efficiency is measured in terms of number of plates which is

directly related to the square of the peak width
inversely related to the square of the peak width
directly related to the square of the peak width
inversely related to the cube root of the peak width

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HPLC
Void volume refers to the

the volume of the column between the point at which solvents are mixed and the beginning of the column
total volume of eluent in the column the remainder being taken up by the packing material
the time required for the gradient to reach the column
the volume of solvent contained in a liquid chromatographic column

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HPLC
Dwell volume is defined as

the volume of the column between the point at which solvents are mixed and the beginning of the column
none of these
the time required for the gradient to reach the column
the volume of solvent contained in a liquid chromatographic column

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