HPLC
An isocratic elution in HPLC is one in which the composition of the solvent

remains constant
changes continuously
none of these
changes in a series of steps

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HPLC
HPLC methods include

liquid/solid (adsorption) chromatography
liquid/liquid (partition) chromatography
All of these
ion exchange and size exclusion chromatography

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HPLC
An eluotropic series

ranks column packing material by their relative abilities to retain solutes on the column
None of these
ranks solvents by their relative abilities to displace solutes from a given absorbent
is a measure of the solvent adsorption energy

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HPLC
Void volume refers to the

the time required for the gradient to reach the column
the volume of the column between the point at which solvents are mixed and the beginning of the column
total volume of eluent in the column the remainder being taken up by the packing material
the volume of solvent contained in a liquid chromatographic column

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