HPLC
A gradient elution in HPLC is one in which the composition of the solvent

remains constant
is changed continuously or in a series of steps
None of these
both (a) and (b)

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HPLC
HPLC stands for

High Performance Liquid Chromatography
both (a) and (b)
Highly Placed Liquid Chromatography
High Pressure Liquid Chromatography

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HPLC
Void volume refers to the

total volume of eluent in the column the remainder being taken up by the packing material
the time required for the gradient to reach the column
the volume of the column between the point at which solvents are mixed and the beginning of the column
the volume of solvent contained in a liquid chromatographic column

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