DNA Replication
Which of the following enzymes unwind short stretches of DNA helix immediately ahead of a replication fork?

DNA polymerases
Topoisomerases
Helicases
Single-stranded binding proteins

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DNA Replication
Proofreading activity to maintain the fidelity of DNA synthesis

is a function of the 3'-5' exonuclease activity of the DNA polymerases
occurs in prokaryotes but not eukaryotes
requires the presence of an enzyme separate from the DNA polymerases
occurs after the synthesis has been completed

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