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Cell Cultivations

Cell Cultivations
Thiobacillus thiooxidans has an optimum pH of

2.0-3.5
9.0-9.5
0.5-6.0
6.5-7.5

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Cell Cultivations
Stringent anaerobes can be grown in a media by taking special measure as

addition of cysteine
any of these
passing through oxygen-free nitrogen
boiling the media for several minutes

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Cell Cultivations
The cell mass can be measured optically by determining the amount of light scattered by a suspension of cells. The measurements are usually at a wavelength of

600-700nm
400-500nm
500-600nm
300-400nm

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Cell Cultivations
For most bacteria, the optimum pH for growth lies between

6.5-7.5
3.5-4.5
4.5-5.5
5.5-6.5

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Cell Cultivations
Semi-solid media, prepared with agar at concentrations of 0.5% or less are useful for the cultivation of

Microaerophillic bacteria
Lactobacilli
all of these
E.coli

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Cell Cultivations
Radical shifts in pH can be prevented by incorporating

a reducing agent
any of these
a buffer
an oxidizing agent

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