Protein Synthesis
Translation in prokaryotes begins by the formation of a 30S initiation complex between the

30S ribosomal subunit, mRNA, initiation factors and N-fMet tRNA
30S ribosomal subunit, tRNA and initiation factors
30S ribosomal subunit, mRNA and initiation factors
30S ribosomal subunit and mRNA only

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Protein Synthesis
The direction of amino acid transfer to the growing polypeptide chain is

from the P site to the E site on the ribosome
from the A (aminoacyl tRNA site) site to the P (peptidyl tRNA site) site on the ribosome
from the P site to the A site on the ribosome
from the A site to the E (exit tRNA site) site on the ribosome

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