Bacteria Morphology
The next to last step in peptidoglycan biosynthesis is

cross-linking the peptide side chains of peptidoglycan
synthesis of the NAM-peptide subunit
removal of the subunit from bactoprenol
linking the sugar of the disaccharide-peptide unit to the growing peptidoglycan chain

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Bacteria Morphology
Gram positive cells have a

periplasmic space that traps the crystal violet
thick capsule that traps the crystal violet stain
second outer membrane that helps to retain the crystal violet stain
multiple layer of peptidoglycan that helps to retain the crystal violet stain

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