Bacteria Morphology
The last step in synthesis of peptidoglycan is

binding of penicillin to a membrane protein
attachment of a portion of peptidoglycan to a membrane lipid
attachment of a peptide to muramic acid
attaching two amino acids to form a cross-link

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Bacteria Morphology
Gram positive cells have a

multiple layer of peptidoglycan that helps to retain the crystal violet stain
periplasmic space that traps the crystal violet
second outer membrane that helps to retain the crystal violet stain
thick capsule that traps the crystal violet stain

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Bacteria Morphology
The next to last step in peptidoglycan biosynthesis is

linking the sugar of the disaccharide-peptide unit to the growing peptidoglycan chain
synthesis of the NAM-peptide subunit
removal of the subunit from bactoprenol
cross-linking the peptide side chains of peptidoglycan

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