Microbial Recombination and Gene Transfer
The expression of gene X (which has promoter Px) is to be monitored. A gene fusion construction for carrying this work will

give the same information as from a microarray
have the promoter of lacZ or some other reporter gene
allow to monitor the expression of all genes with a promoter similar in sequence to Px
have Px but not the rest of the X coding region

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Microbial Recombination and Gene Transfer
The main difference between a self-transmissible and a mobilizable plasmid is that the self-transmissible plasmid

usually has a transposon inserted into it
carries genes encoding the mating apparatus
transfers both strands of the plasmid DNA
transfers antibiotic resistance genes

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Microbial Recombination and Gene Transfer
In lysogeny,

a bacteriophage transfers bacterial DNA
bacteria take up double stranded DNA from the environment
a bacteriophage genome is integrated into the bacterial genome
DNA-degrading enzymes in the extracellular medium would stop the process

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