Carbohydrate
The conversion of pyruvate to oxaloacetate

requires biotin
involves the fixation of carbon dioxide
occurs in the mitochondria
all of the above

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Carbohydrate
When living organisms are cooled below some critical body temperature, the metabolic reactions within their cells cease to function properly. This malfunction occurs because

enzyme active sites become permanently bound to substrates
their enzymes lose the proper three-dimensional shape
the activation energy for the reaction is raised
there is insufficient molecular motion for substrates to interact

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