HPLC QUIZ

non polar solvent/polar column

polar solvent/non-polar column

any of the above

non polar solvent/non-polar column

changes continuously

none of these

remains constant

changes in a series of steps

100 and 150 A°

10 and 50 A°

150 and 200 A°

60 and 100 A°

the volume of solvent contained in a liquid chromatographic column

total volume of eluent in the column the remainder being taken up by the packing material

the time required for the gradient to reach the column

the volume of the column between the point at which solvents are mixed and the beginning of the column

the volume of the column between the point at which solvents are mixed and the beginning of the column

the time required for the gradient to reach the column

none of these

the volume of solvent contained in a liquid chromatographic column

All of these

liquid/solid (adsorption) chromatography

ion exchange and size exclusion chromatography

liquid/liquid (partition) chromatography

None of these

ranks column packing material by their relative abilities to retain solutes on the column

ranks solvents by their relative abilities to displace solutes from a given absorbent

is a measure of the solvent adsorption energy

Any compound with solubility in liquid

Any compound with non-solubility in liquid

Any of the above

Any compound with limited solubility in liquid

non polar solvent/non-polar column

polar solvent/non-polar column

non polar solvent/polar column

any of the above

All of these

Greater sensitivity and reusable columns

Sample recovery

Ideal for ionic species and large molecules