HPLC QUIZ

High Pressure Liquid Chromatography

High Performance Liquid Chromatography

both (a) and (b)

Highly Placed Liquid Chromatography

total volume of eluent in the column the remainder being taken up by the packing material

the volume of the column between the point at which solvents are mixed and the beginning of the column

the time required for the gradient to reach the column

the volume of solvent contained in a liquid chromatographic column

Greater sensitivity and reusable columns

Sample recovery

All of these

Ideal for ionic species and large molecules

changes continuously

remains constant

none of these

changes in a series of steps

solvent absorption energy

solvent mixing index

solvent adsorption energy

solvent diffusivity

non polar solvent/polar column

non polar solvent/non-polar column

any of the above

polar solvent/non-polar column

liquid/liquid (partition) chromatography

liquid/solid (adsorption) chromatography

ion exchange and size exclusion chromatography

All of these

both (a) and (b)

is changed continuously or in a series of steps

None of these

remains constant

non polar solvent/polar column

polar solvent/non-polar column

any of the above

non polar solvent/non-polar column

directly related to the square of the peak width

inversely related to the square of the peak width

inversely related to the cube root of the peak width

directly related to the square of the peak width