Plant Cell Culture
Protoplasts can be produced from suspension cultures, callus tissues or intact tissues by enzymatic treatment with

cellulotyic enzymes
both cellulotyic and pectolytic enzymes
proteolytic enzymes
pectolytic enzymes

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Plant Cell Culture
In a callus culture

only auxin is required for root and shoot formation
increasing level of cytokinin to a callus induces shoot formation and increasing level of auxin promote root formation
auxins and cytokinins are not required
increasing level of auxin to a callus induces shoot formation and increasing level of cytokinin promote root formation

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