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Animal Breeding and Transgenic Animal

Animal Breeding and Transgenic Animal
In transgenic fish, the genes are introduced by

all of these
transfer of whole nuclei
microinjection in fish
viruses

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Animal Breeding and Transgenic Animal
Chromosomes may be isolated from metaphase cells by

isotonic lysis
either (a) or (b)
hypertonic lysis
hypotonic lysis

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Animal Breeding and Transgenic Animal
The nucleous of mature unfertilized ovum may be removed by

irradiation
both (a) and (b)
neutralization and homogenization
surgery

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Animal Breeding and Transgenic Animal
Karyoplast is

nuclei
nuclei with only some residual plasma membrane
cell with nucleus
cells devoid of cell wall

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Animal Breeding and Transgenic Animal
The success of embryo transplantation depends upon the ability to obtain an increased number of embryos from animals of superior genetic merit, which can be achieved by the use of

both (a) and (b)
FSH (Follicle Stimulating Hormone)
endogenous gonadatrophic hormones
exogenous gonadatrophic hormones

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Animal Breeding and Transgenic Animal
Enucleation of the cells can be achieved by treating the cells with

both (a) and (b)
polyethylene glycol
alcohol
cytochalasin B

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