Microbial Recombination and Gene Transfer
In the extracellular medium, DNA-degrading enzymes would likely be to prevent transfer of DNA by

conjugal transfer by a self-transmissible plasmid
natural transformation
generalized phage transduction
None of these

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Microbial Recombination and Gene Transfer
Which of the following statement(s) is/are true in regards to F+ x F- mating events?

No DNA is transferred because F+ cells are unable to perform conjugation
No DNA is transferred because F- cells are unable to perform conjugation
DNA is transferred from F+ to F- cells
DNA is transferred from F- to F+ cells

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Microbial Recombination and Gene Transfer
The expression of gene X (which has promoter Px) is to be monitored. A gene fusion construction for carrying this work will

allow to monitor the expression of all genes with a promoter similar in sequence to Px
have Px but not the rest of the X coding region
have the promoter of lacZ or some other reporter gene
give the same information as from a microarray

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