Carbohydrate
Gluconeogenesis requires a higher amount of ATP equivalents as compared to that produced by glycolysis because

glycolysis occurs in the mitochondria while gluconeogenesis occurs in the cytosol
gluconeogenesis releases energy as heat
glycolysis releases energy as heat
All of these

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Carbohydrate
When living organisms are cooled below some critical body temperature, the metabolic reactions within their cells cease to function properly. This malfunction occurs because

enzyme active sites become permanently bound to substrates
the activation energy for the reaction is raised
there is insufficient molecular motion for substrates to interact
their enzymes lose the proper three-dimensional shape

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