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Cell Cultivations

Cell Cultivations
For most bacteria, the optimum pH for growth lies between

3.5-4.5
6.5-7.5
5.5-6.5
4.5-5.5

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Cell Cultivations
Callus cultures are amorphous cell aggregates arising from the unorganized growth of explants on an

solid nutrient medium
aseptic solid nutrient medium
liquid medium
solid or liquid medium

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Cell Cultivations
Semi-solid media, prepared with agar at concentrations of 0.5% or less are useful for the cultivation of

E.coli
Microaerophillic bacteria
Lactobacilli
all of these

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Cell Cultivations
The isolation of the gonorrhoea - causing organisms, Neisseria gonorrhoeae,from a clinical specimen is facilitated by the use of media containing

none of these
succinate
cellulose
certain antibiotics

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Cell Cultivations
Anchorage -dependent cells require

either (a) or (b)
wettable surface for growth
dry surface for growth
nothing to do with the surface

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Cell Cultivations
Thiobacillus thiooxidans has an optimum pH of

9.0-9.5
0.5-6.0
6.5-7.5
2.0-3.5

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