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Cell Cultivations

Cell Cultivations
For most bacteria, the optimum pH for growth lies between

3.5-4.5
5.5-6.5
4.5-5.5
6.5-7.5

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Cell Cultivations
Thiobacillus thiooxidans has an optimum pH of

9.0-9.5
6.5-7.5
0.5-6.0
2.0-3.5

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Cell Cultivations
Radical shifts in pH can be prevented by incorporating

a buffer
a reducing agent
any of these
an oxidizing agent

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Cell Cultivations
Callus cultures are amorphous cell aggregates arising from the unorganized growth of explants on an

solid or liquid medium
aseptic solid nutrient medium
solid nutrient medium
liquid medium

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Cell Cultivations
The size of a single plant cell is usually within the range of

60-80μm in diameter and 300-400μm long
40-60μm in diameter and 200-300μm long
20-40μm in diameter and 100-200μm long
10-20μm in diameter and 25-100μm long

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Cell Cultivations
Anchorage -dependent cells require

nothing to do with the surface
wettable surface for growth
either (a) or (b)
dry surface for growth

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