Bacteria Morphology
The next to last step in peptidoglycan biosynthesis is

linking the sugar of the disaccharide-peptide unit to the growing peptidoglycan chain
removal of the subunit from bactoprenol
synthesis of the NAM-peptide subunit
cross-linking the peptide side chains of peptidoglycan

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Bacteria Morphology
Gram positive cells have a

periplasmic space that traps the crystal violet
multiple layer of peptidoglycan that helps to retain the crystal violet stain
second outer membrane that helps to retain the crystal violet stain
thick capsule that traps the crystal violet stain

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